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目的:研究悬吊废用和外源性血管紧张素Ⅱ对大鼠骨骼肌运动终板形态和功能的影响。方法:雌性SD大鼠32只,体重(200~220)g,分成4组,每组8只,包括安静对照组(C)、安静加血管紧张素组(CA)、尾部悬吊组(T)、悬吊加血管紧张素组(TA)。T和TA组采用尾悬吊造成腓肠肌的废用萎缩。CA和TA组利用缓释泵稳定地对体内释放血管紧张素Ⅱ(AngⅡ)。取材后,氯化金(AuCl3)染色法显示运动终板的形态;乙酰胆碱酯酶(AchE)染色法显示AchE的相对含量;Western blot法测定神经营养因子-3(NT-3)的相对含量。结果:与C组相比,T组运动终板核面积和AchE相对含量均显著性下降(P<0.01),而TA组两指标比T组有显著性的提高(P<0.01);与C组相比,CA组NT-3表达显著性升高(P<0.01),而T组NT-3的表达显著性下降(P<0.01);TA组NT-3显著高于T组(P<0.05)。结论:悬吊废用会导致运动神经末梢和运动终板发生退化;AngⅡ有可能通过神经营养因子的作用阻止肌肉运动终板的退化。
Abstract：Objective: To study the effect of tail suspension and angiotensin Ⅱ on the structure and function of motor end plate in rats.Methods: 32 female SD rats,200~220 g,were divided into 4 groups,8 per group,control group(C),angiotensinⅡ group(CA),tail suspension group(T),tail suspension and angiotensin Ⅱ group(TA).Atrophy of gastrocnemius induced by tail suspension.Angiotensin Ⅱ released by Alzet Osmotic Pumps to the rats constantly.AuCl3 staining method showed the changes of motor end plate structure.AchE staining examined the changes of AchE.Western blot analyzed the expression of neurotrophins-3(NT-3).Results: The area of motor end plate and the IODof AchE of group T decreased significantly(P<0.01) compared with group C.The area of motor end plate and the IOD of AchE of group TA had a significant increase than group T(P<0.01).The expression of NT-3 of group CA had a significantly increased(P<0.01),and group T decreased(P<0.01) compared with group C.The NT-3 expression of group TA increased significantly than group T(P<0.05).Conclusions: Suspension of disuse can lead to motor end plate degenerated,and the AngⅡ maybe inhibit the muscle atrophy and motor end plate degeneration by the NT-3.
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基本信息:
DOI：10.13297/j.cnki.issn1005-0000.2012.02.002
中图分类号:G804.2
引用信息:
[1]周越,王兵.悬吊废用和血管紧张素Ⅱ对大鼠骨骼肌运动终板的影响[J].天津体育学院学报,2012,27(02):158-161.DOI:10.13297/j.cnki.issn1005-0000.2012.02.002.
基金信息:
教育部高等学校博士点基金资助课题(项目编号:20070043008)