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目的:观察疲劳训练对大鼠学习记忆能力、海马氨基酸含量及海马CA1、CA3区c-fos表达的影响,探讨疲劳训练降低大鼠学习记忆能力的可能机制。方法:将30只SD大鼠(3月龄)随机分为对照组、有氧训练组及疲劳训练组,有氧训练组采用中等负荷跑台运动,疲劳训练组采用递增负荷疲劳训练,连续8周。8周训练结束后,第2天利用水迷宫实验测试大鼠空间学习记忆能力,水迷宫行为测试结束后,即刻检测大鼠海马氨基酸含量,采用免疫组织化学结合图像半定量方法对海马CA1、CA3区c-fos神经元表达进行测量和分析。结果:(1)与对照组比较,有氧训练组及疲劳训练组大鼠海马谷氨酸含量均增高(P<0.05,P<0.01),有氧训练组大鼠GABA含量与对照组的差异无统计学意义(P>0.05),Glu/GABA比值显著增高(P<0.05),疲劳训练组大鼠GABA含量显著增多(P<0.01),Glu/GABA比值降低(P<0.05);(2)与对照组比较,有氧训练组大鼠逃避潜伏期缩短(P<0.05),穿越平台次数增多(P<0.05),而疲劳训练组大鼠逃避潜伏期延长(P<0.01),穿越平台次数减少(P<0.05);(3)8周跑台运动后,有氧训练组及疲劳训练组大鼠CA1、CA3区c-fos神经元的数量及面积均较对照组明显增加(P<0.05,P<0.01)。结论:海马谷氨酸过度分泌导致CA1、CA3区c-fos神经元过度表达,可能参与了运动疲劳导致的大鼠学习记忆能力的下降。
Abstract：Objective:Observing the influence of fatigue training on learning and memory,Hippocampal amino acid content and c-fos expression in the Hippocampus CA1,CA3 of the fatigue training rats and inquiring into the mechanism of the fatigue training decreasing learning and memory. Methods :30 SD rats(3 months)were chosen and randomly divided into 3 groups,which included control group(C),aerobic training group(AT)and fatigue training group(FT). The C group rats kept normal activities,AT group rats undertook a 8-week moderate-load treadmill training and FT group rats undertook a 8-week fatigue training with progressively increasing load. The morris water maze test was used to assess each animal's capability of learning and memory the next day after 8-week treadmill training,then hippocampal amino acid content were examined,c-fos expression in hippocampus CA1,CA3 was detected and analyzed by using ABC immuneohistochemical technique and semi quantitative. Results:(1)Compared with the C group,Glu content in hippocampal significantly increased(P<0.05,P<0.01)in the AT and FT group rats;GABA content in hippocampal was not significant difference(P>0.05)and the ratio of Glu/GABA increased in AT group rats;GABA content and the ratio of Glu/GABA in hippocampal significantly increased(P<0.05,P<0.01)in FT group rats when compared to the C group rats(.2)Compared with the C group,escape latency shortened(P<0.05),the times across platform increased(P<0.05)in AT group rats,but escape latency extened(P<0.01)and the times across platform decreased(P<0.05)in FT group rats;(3)Compared with the C group,the number and area of cfos in hippocampus CA1,CA3 notably increased in the AT and FT group rats(P<0.05,P<0.01). Conclusion:Glu over secretion in hippocampus causing c-fos over expression in hippocampus CA1,CA3 may contribute to the learning and memory declining in fatigue training rats.
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基本信息:
DOI：10.13297/j.cnki.issn1005-0000.2013.05.010
中图分类号:G804.2
引用信息:
[1]唐伟,王霆.海马氨基酸参与调控运动性疲劳大鼠学习记忆能力及海马CA1、CA3区c-fos的表达[J].天津体育学院学报,2013,28(05):414-418.DOI:10.13297/j.cnki.issn1005-0000.2013.05.010.
基金信息:
国家体育总局奥运攻关项目(项目编号:10A011)