| 53 | 0 | 82 |
| 下载次数 | 被引频次 | 阅读次数 |
目的:本研究的第一个目的是模拟在血液收集和分析场所之间针对样品转移可选择的方法,第二个目的是对比同一样本在不同的天数时所得的分析结果。方法:26名健康男性(年龄20～50岁)志愿提供血液样本。血液离心后得到的血清,被分成7部分。每一部分都被单独处理以模拟不同的转移方法和存储条件。随后分析每一部分中的乳酸脱氢酶(LDH),乳酸脱氢酶同工酶1(LDH-1),肌酸激酶(CK)和肌酸激酶心型同工酶(CK-MB)的活性。结果:CK和CK-MB在基础值和其他样本中所得结果没有显著性差异(P>0.05);LDH和LDH-1也有相似的结果,只是在模拟不带冰转移时,其结果显著高于基础值(P<0.05)。结论:采用带冰的容器转移血液样本,然后冷冻。这样,即使推迟5天测量,其结果也不会受到影响。
Abstract：Purpose;The first objective of this study was to simulate the options available to transfer blood between the collection and analysis venues.The sec- ond objective was to compare the results obtained from analysing the same sample on different days.Methods:Twenty six healthy men(age range 20-50 years)gave informed consent to provide blood samples.After centrifuging,the obtained serum was split into seven portions and each portion was treated in- dependently to simulate different modes of transfer and different storage conditions.Each portion was then analysed for the activities of lactate dehydroge- nase(LDH),lactate dehydrogenase isoenzyme 1(LDH-1),creatine kinase(CK)and creatine kinase polypeptide sub-unit MB(CK-MB).Results:The results showed that there was a non-significant difference between the baseline values and the values in other samples for CK and CK-MB(P>0.05).LDH and LDH-1 showed similar results except with the simulated transfer without ice in which the value was significantly greater than that of the baseline(P<0.05). Conclusion:It can therefore be stated with confidence that delaying the measurement by up to five days following freezing,having transported the blood samples in an ice box will not affect the results.
[1]Linseman D A,Wall T M,Hartman J C.The trolox analog,U-78517F, attenuates hypoxic injury in isolated cardiac myocytes[J].journal of Molecular and Cellular Cardiology,1994,26:1 249-1 257.
[2]Moreau D,Dubots P,Bnggio V,et al.Effects of electromyostimulation and strength training on muscle soreness,muscle damage and sympathetic activation[J].Journal of Sports Sciences,1995,13;95-100.
[3]Anderson J L,Karagounis L A,Califf R M.Metaanalysis of five reported studies on the relation of early coronary potency grades with mortality and outcomes after acute myocardial infarction[J].The American Journal of Cardiology,1996,78(1):1-8.
[4]Bredie D A,Liu X,Jackson M.Changes in serum biochemical responses during cardiac rehabilitation[J].Medicine and Science in Sports and Exercise,2003,35(5);741-746.
[5]Urso S,Milone F,Garozzo M,et al.Cardiovaacular risk markers in hemodial- ysis[J].Giornale Italiano di Nefrologia,2004,21(Suppl30);S212-S216.
[6]Bergrneyer H U,Bergmeyer J,Grail M.Methods of enzymatic analysis (3rd Ed.).Vol.ⅢEnzymes 1:oxidoreductases,transferases[M].Verlag Chemie,Weinheim,1983.
[7]Liu T Z,Wei J S.Diagnostic efficacy of a moneclonal antibody-based im- munoenzymetric technique for quantitation of creatine kinase MB isoen- zyme in serum[J].Journal of Formosan Medical Association,1990,89(4): 259-263.
[8]Adan J,Bernstein L H,Babb J.Lactate dehydrogenaso isoenzyme-1/total ratio;Accurate for determining the existence of myocardial infarction[J]. Clinical Chemistry,1986,32(4);624-628.
[9]Ohman E M,Casey C,Bengtson J R,et al.Early detection of acute my- ocardial infarction;additional diagnostic information from serum concen- trations of myoglobin in patients without ST elevation[J].British Heart Journal,1990,63;335-338.
[10]Lutoslawska G,Sendecki W.Plasma biochemical variables in response to 42-km Kayak and canoe races[J].The Journal of Sports Medicine and Physical Fitness,1990,30:406-411.
[11]Rotenberg Z,Weinberger I,Sagie A,et al.Lactate dehydorgenase isoen- zymes in serum during recent acute myocardial infarction[J].Clinical Chemistry,1987,33(8);1 419-1 420.
[12]Wu AHB,Bowers GN Jr.Evaluation and comparison of immunoinhibi- tion and immunoprecipitation methods for differentiating MB and BB and macro form of creatine kinase isoenzymes in patients and healthy in- dividuals[J].Clinical Chemistry,1982,28:10:2 017-2 021.
[13]Auer J,Berent R,Labetanig E,et al.Serum neopterin and activity of coronary artery disesso[J].Heart Disease,2001,3(5):297-301.
[14]Despres J P,Gagnon J,Bergeron J,et al.Plasma post-heparin lipase ac- tivities in the HERITAGE Family Study;the reproducibility,gender dif- ferences,and associations with lipoprotein levels.Health,Risk factors, exercise Training and Genetics[J].Clinical Biochemistry,1999,32(3): 157-65.
[15]Castilla J A,Alvarez C,Aguilar J,et al.Influence of analytical and bio- logical variation on the clinical interpretation of seminal parameters[J]. Human Reproduction,2006,21(4);847-51.
基本信息:
中图分类号:G804.2
引用信息:
[1]刘洵,Brodie DA,金川,等.转移方法和推迟分析对酶活性的影响(英文)[J].天津体育学院学报,2007(06):468-471.
基金信息:
天津市高等学校科技发展基金(项目编号：2005xk22)