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目的:探讨抗阻训练对衰老小鼠心肌sarcopenia中Akt/mTOR信号通路的影响。方法:雄性,3月龄SAMP8小鼠随机分为对照组(n=12)和抗阻训练组(n=12)。对照组常规喂养至6月龄,达到衰老后处死;抗阻训练组进行3个月的抗阻训练,建立衰老小鼠抗阻训练运动模型,使用实时荧光定量PCR技术,对小鼠心肌Akt/mTOR信号通路中Akt、mTOR、p70S6K和eIF-4E基因mRNA的表达进行测定。结果:衰老小鼠出现心肌纤维数目减少,心肌纤维空白区域面积增多;抗阻训练后,小鼠心脏重量和心系数与衰老对照组相比虽然显著增加(P<0.05)但没有产生病理性心肌肥大;心肌纤维空白区域面积小于对照组;抗阻训练组Akt、mTOR、p70S6K和eIF-4E基因mRNA的表达与衰老对照组相比显著上升(P<0.05)。结论:抗阻训练可以增强衰老小鼠心肌Akt/mTOR信号通路中各基因mRNA的表达,产生运动性心肌肥大,并对心肌产生保护性调节作用,改善衰老小鼠的心脏功能。
Abstract：Objective:To study the effect of resistance training on cardiac sarcopenia.Methods:Male SAMP8 3-months old mice were randomly divided into control group(n=12)and resistance training group(n=12).The control group was executed at 6-months old.After inducing the animal model of resis-tance training,using the realtime PCR system to analyze the mRNA expression of Akt,mTOR,p70S6K and eIF-4E in mouse heart.Result:The aging mice turned cardiac sarcopenia.After resistance training,the mouse heart weight and the ventricular mess index significantly increased(P<0.05),without pathological cardiac hypertrophy,and did not turn cardiac sarcopenia.The mRNA expressions of Akt,mTOR,p70S6K and eIF-4E of resistance training group increased significantly than that of the aging control group(P<0.05).Conclusion:The resistance training can elevate the cardiac PI3-K/Akt signaling pathway expression,induce exercise cardiac hypertrophy,start the cardiac cell protection control action,and improve the cardiac function of aging mice.
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基本信息:
中图分类号:G804.7
引用信息:
[1]李欣,丁树哲,卢健.抗阻训练对衰老小鼠心肌Sarcopenia中Akt/mTOR信号通路的影响[J].天津体育学院学报,2009,24(04):334-336+365.
2009-07-25
2009-07-25