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目的:在NAD合成酶基因表达改变后的遗传背景下,研究不同年龄段进行规律运动干预对果蝇生命周期和快速攀爬能力的影响,为研究NAD合成酶基因的运动抗衰老效应以及训练效应提供更全面的理论依据。方法:利用UAS/GAL4系统使果蝇NAD合成酶基因表达正常、下调、上调和突变,不同遗传背景果蝇分为对照组、运动6周组、7周后运动组和终身运动组,利用转动平台对不同时期果蝇进行运动训练,q RT-PCR检测NAD合成酶基因m RNA表达量,测量果蝇攀爬速度和统计生命周期。结果:表达下调组果蝇CG9940 m RNA表达量低于正常表达组(P<0.01),下调率为56.98%;表达上调组高于正常表达组(P<0.01),上调率为129.21%;突变组表达量低于正常表达组(P<0.01)。CG9940表达下调组果蝇寿命低于正常表达组(P<0.05),表达上调组和突变组寿命高于正常表达组(P<0.05,P<0.01);正常表达组和表达上调组果蝇的运动6周、7周后运动、终身运动组寿命高于安静组(P<0.05,P<0.01,P<0.05;P<0.01,P<0.01,P<0.05);表达下调的运动6周组寿命低于安静组(P<0.01);突变的运动6周组、终身运动组寿命低于安静组(P<0.01,P<0.01)。正常表达组和表达上调组3周龄果蝇的攀爬速度高于安静组(P<0.05,P<0.05)。结论:果蝇NAD合成酶基因CG9940表达不同的群体中,生命晚期开始的适宜规律运动能对更广泛群体的寿命产生效益;CG9940基因表达上调的攀爬效应能与生命早期训练的攀爬效应产生叠加,提高攀爬能力。果蝇CG9940功能与生命周期、攀爬能力密切相关,不同个体的运动抗衰老效应、运动的攀爬效应可能与NAD合成酶基因表达及突变有关。
Abstract：Objective:Constructed CG9940 expression decreasing,raising and mutation,to studies The effects of regular exercise begins at different agescombined CG9940 gene polymorphism on fast climbing ability and lifespan in drosophila. Methods:used the UAS/GAL4 system to make CG9940 gene expres-sion changes after flies crossing,rotating platform is used to training at different age stages of fruit flies,detected CG9940 gene m RNA expression by q-RT-PCR,measured climbing speed and lifespan statistics of fly. Results:Compared with the relative expression of wild-type flies,NAD synthetase gene down-regulation fruit flies m RNA expression expression decreased(P<0.01),lower rate of 56.98%;over-xpression fruit flies relatively increased expression(P<0.01),increase rate of 129.21%;mutation expression amount is very low,and the normal expression group differ about 8.70 times. compared with wild-typeflies,climbing speed increased after raised CG9940 gene expression on young(P<0.01)and elderly(P<0.05)fruit flies,Mutant enhanced climbing speed onyoung(P<0.01)and elderly(P<0.01)drosophila,at 3rd week,control group climbing speed is lower than the exercise group in both in wild-type flies(P<0.05)and CG9940 over-expression flies(P<0.05). Compared with heart fibrillation index in wild-type flies,decreased the CG9940 gene expression in-creased fibrillation index(P<0.05),over-expression's is lower(P<0.05),mutant's is lower(P<0.01);Compared with wild-type flies,CG9940 gene ex-pression reduced makes lifespan shorten(P<0.05),over-expression and mutant extend the lifespan(P<0.05,P<0.01),exercising 6 weeks,trained after the6 th week,lifelong exercised prolong lifespan of the wild type(P<0.05,P<0.01,P<0.05)and over-expression CG9940 gene flies(P<0.01,P<0.01,P<0.05),exercising 6 weeks shorten lifespan in CG9940 gene expression decreased drosophila(P<0.01),exercising 6 weeks and lifetime training shorten lifespan inmutan(tP<0.01,P<0.01). Conclusion:Under the different genetic background of CG9940 gene,exercise has different effects on fast climbing,only wild andover-expression flies' fast climbing benefit from early exercise;Training begins at different ages show the different influences on lifespan,but exercise afterthe 6th week prolong life widely. Suggesting NAD synthetase gene expression can be used as one of athlete gene selection index;Older individuals should par-ticipate in appropriate exercise.
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基本信息:
DOI：10.13297/j.cnki.issn1005-0000.2016.04.012
中图分类号:R87
引用信息:
[1]文登台,郑澜,李秋芳,等.不同年龄段规律运动联合NAD合成酶基因CG9940表达改变对果蝇生命周期和运动能力的影响[J].天津体育学院学报,2016,31(04):340-345+350.DOI:10.13297/j.cnki.issn1005-0000.2016.04.012.
基金信息:
教育部博士点基金资助项目(项目编号:20134306110009);; 湖南省研究生科研创新项目(项目编号:CX2016B174)
2016-07-25
2016-07-25